|Title||Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Fislage, M., M. Roovers, S. Münnich, L. Droogmans, and W. Versées|
|Journal||Acta Crystallogr Sect F Struct Biol Cryst Commun|
|Date Published||2011 Nov 01|
|Keywords||Crystallization, Crystallography, X-Ray, Pyrococcus furiosus, Thermus thermophilus, tRNA Methyltransferases|
Methyltransferases form a major class of tRNA-modifying enzymes that are needed for the proper functioning of tRNA. Here, the expression, purification and crystallization of two related putative tRNA methyltransferases from two kingdoms of life are reported. The protein encoded by the gene pf1002 from the archaeon Pyrococcus furiosus was crystallized in the monoclinic space group P2(1). A complete data set was collected to 2.2 Å resolution. The protein encoded by the gene ttc1157 from the eubacterium Thermus thermophilus was crystallized in the trigonal space group P3(2)21. A complete data set was collected to 2.05 Å resolution.
|Alternate Journal||Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.|
|PubMed Central ID||PMC3212469|
Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus.