Molecular basis of gyrase poisoning by the addiction toxin CcdB.

TitleMolecular basis of gyrase poisoning by the addiction toxin CcdB.
Publication TypeJournal Article
Year of Publication2005
AuthorsDao-Thi, M-H., L. Van Melderen, E. De Genst, H. Afif, L. Buts, L. Wyns, and R. Loris
JournalJ Mol Biol
Date Published2005 May 20
KeywordsBacterial Proteins, Bacterial Toxins, Catalytic Domain, Crystallography, Dimerization, DNA Gyrase, Escherichia coli, F Factor, Molecular Structure, Mutagenesis, Site-Directed, Mutation, Protein Structure, Tertiary

Gyrase is an ubiquitous bacterial enzyme that is responsible for disentangling DNA during DNA replication and transcription. It is the target of the toxin CcdB, a paradigm for plasmid addiction systems and related bacterial toxin-antitoxin systems. The crystal structure of CcdB and the dimerization domain of the A subunit of gyrase (GyrA14) dictates an open conformation for the catalytic domain of gyrase when CcdB is bound. The action of CcdB is one of a wedge that stabilizes a dead-end covalent gyrase:DNA adduct. Although CcdB and GyrA14 form a globally symmetric complex where the two 2-fold axes of both dimers align, the complex is asymmetric in its details. At the centre of the interaction site, the Trp99 pair of CcdB stacks with the Arg462 pair of GyrA14, explaining why the Arg462Cys mutation in the A subunit of gyrase confers resistance to CcdB. Overexpression of GyrA14 protects Escherichia coli cells against CcdB, mimicking the action of the antidote CcdA.

Alternate JournalJ. Mol. Biol.
PubMed ID15854646