Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose.

TitleCrystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose.
Publication TypeJournal Article
Year of Publication2003
AuthorsLoris, R., A. Imberty, S. Beeckmans, E. Van Driessche, J. S. Read, J. Bouckaert, H. De Greve, L. Buts, and L. Wyns
JournalJ Biol Chem
Volume278
Issue18
Pagination16297-303
Date Published2003 May 2
ISSN0021-9258
KeywordsBinding Sites, Crystallization, Disaccharides, Glucose, Molecular Conformation, Plant Lectins, Pterocarpus, Sucrose
Abstract

The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site.

DOI10.1074/jbc.M211148200
Alternate JournalJ. Biol. Chem.
PubMed ID12595543