Title | Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Loris, R., A. Imberty, S. Beeckmans, E. Van Driessche, J. S. Read, J. Bouckaert, H. De Greve, L. Buts, and L. Wyns |
Journal | J Biol Chem |
Volume | 278 |
Issue | 18 |
Pagination | 16297-303 |
Date Published | 2003 May 2 |
ISSN | 0021-9258 |
Keywords | Binding Sites, Crystallization, Disaccharides, Glucose, Molecular Conformation, Plant Lectins, Pterocarpus, Sucrose |
Abstract | The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site. |
DOI | 10.1074/jbc.M211148200 |
Alternate Journal | J. Biol. Chem. |
PubMed ID | 12595543 |
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