|Title||The NMR determination of the IIA(mtl) binding site on HPr of the Escherichia coli phosphoenol pyruvate-dependent phosphotransferase system.|
|Publication Type||Journal Article|
|Year of Publication||1993|
|Authors||van Nuland, N. A. J., G. J. Kroon, K. Dijkstra, G. K. Wolters, R. M. Scheek, and G. T. Robillard|
|Date Published||1993 Jan 2|
|Keywords||Bacterial Proteins, Binding Sites, Escherichia coli, Macromolecular Substances, Magnetic Resonance Spectroscopy, Phosphoenolpyruvate Sugar Phosphotransferase System, Phosphorylation, Protein Structure, Tertiary|
The region of the surface of the histidine-containing protein (HPr) which interacts with the A domain of the mannitol-specific Enzyme II (II(Amt1)) has been mapped by titrating the A-domain into a solution of 15N-labeled HPr and monitoring the effects on the amide proton and nitrogen chemical shifts via heteronuclear single quantum correlation spectroscopy (HSQC). Fourteen of the eighty-five HPr amino acid residues show large changes in either the 15N or 1H chemical shifts or both as a result of the presence of II(Amt1) while a further seventeen residues experience lesser shifts. Most of the residues involved are surface residues accounting for approximately 25% of the surface of HPr. Phosphorylation of HPr with catalytic amounts of Enzyme I (EI), in the absence of II(Amt1) resulted in chemical shift changes in a sub-set of the above residues; these were located more in the vicinity of the active site phospho-histidine. Phosphorylation of the HPr/II(Amt1) complex resulted in a HSQC spectrum which was indistinguishable from the P-HPr spectrum in the absence of II(Amt1) indicating that, as expected, the complex P-HPr/P-II(Amt1) does not exist even at the high concentrations necessary for NMR.
|Alternate Journal||FEBS Lett.|