Introduction to heavy chain antibodies and derived Nanobodies.

TitleIntroduction to heavy chain antibodies and derived Nanobodies.
Publication TypeJournal Article
Year of Publication2012
AuthorsVincke, C., and S. Muyldermans
JournalMethods Mol Biol
Date Published2012
KeywordsAnimals, Antibodies, Antigens, Binding Sites, Antibody, Camelids, New World, Humans, Immunoglobulin Fc Fragments, Immunoglobulin G, Immunoglobulin Heavy Chains, Protein Binding, Single-Domain Antibodies

The immune response of infected or immunized dromedaries contains a diverse repertoire of conventional and heavy chain-only antibodies, both functional in antigen binding. By definition, a heavy chain antibody is devoid of a light chain and in the case of the heavy chain antibodies in camelids the CH1 domain is also missing. Consequently a camelid heavy chain antibody associates with its cognate antigen via a single domain, the variable heavy chain domain of a heavy chain antibody or VHH. An antigen-specific VHH, also known as Nanobody, with excellent biochemical properties can be obtained in various ways. Their recombinant expression provides access to user-friendly tools for a wide variety of applications.

Alternate JournalMethods Mol. Biol.
PubMed ID22886243