Title | Introduction to heavy chain antibodies and derived Nanobodies. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Vincke, C., and S. Muyldermans |
Journal | Methods Mol Biol |
Volume | 911 |
Pagination | 15-26 |
Date Published | 2012 |
ISSN | 1940-6029 |
Keywords | Animals, Antibodies, Antigens, Binding Sites, Antibody, Camelids, New World, Humans, Immunoglobulin Fc Fragments, Immunoglobulin G, Immunoglobulin Heavy Chains, Protein Binding, Single-Domain Antibodies |
Abstract | The immune response of infected or immunized dromedaries contains a diverse repertoire of conventional and heavy chain-only antibodies, both functional in antigen binding. By definition, a heavy chain antibody is devoid of a light chain and in the case of the heavy chain antibodies in camelids the CH1 domain is also missing. Consequently a camelid heavy chain antibody associates with its cognate antigen via a single domain, the variable heavy chain domain of a heavy chain antibody or VHH. An antigen-specific VHH, also known as Nanobody, with excellent biochemical properties can be obtained in various ways. Their recombinant expression provides access to user-friendly tools for a wide variety of applications. |
DOI | 10.1007/978-1-61779-968-6_2 |
Alternate Journal | Methods Mol. Biol. |
PubMed ID | 22886243 |
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