In vitro antiviral activity of single domain antibody fragments against poliovirus.

TitleIn vitro antiviral activity of single domain antibody fragments against poliovirus.
Publication TypeJournal Article
Year of Publication2010
AuthorsThys, B., L. Schotte, S. Muyldermans, U. Wernery, G. Hassanzadeh-Ghassabeh, and B. Rombaut
JournalAntiviral Res
Volume87
Issue2
Pagination257-64
Date Published2010 Aug
ISSN1872-9096
KeywordsAnimals, Antiviral Agents, Camels, HeLa Cells, Humans, Immunoglobulin Fragments, Microbial Sensitivity Tests, Poliovirus, Virus Replication
Abstract

VHHs or Nanobodies are single-domain antigen-binding fragments derived from heavy chain antibodies found in camelids. It has already been shown that complex protein mixtures and even whole organisms elicit good immune responses in camelids; therefore we hypothesized that VHHs selected from a dromedary immunized with poliovirus type 1 might inhibit the in vitro replication of poliovirus through binding to essential biological sites on the viral capsid. In this study, we aimed to determine whether VHHs inhibit wild-type and vaccine strains of poliovirus type 1. Interestingly, VHHs showed a potent antipolio activity with EC50 values in the low nanomolar range. Moreover, these antibody fragments completely blocked viral multiplication at higher concentrations. Remarkably, no (immune) escape variants against some of these VHHs could be generated. In conclusion, VHHs fulfil several in vitro requirements to be assigned as potential antiviral compounds for further development of an anti-poliovirus drugs.

DOI10.1016/j.antiviral.2010.05.012
Alternate JournalAntiviral Res.
PubMed ID20566349