Targeting and tracing antigens in live cells with fluorescent nanobodies.

TitleTargeting and tracing antigens in live cells with fluorescent nanobodies.
Publication TypeJournal Article
Year of Publication2006
AuthorsRothbauer, U., K. Zolghadr, S. Tillib, D. Nowak, L. Schermelleh, A. Gahl, N. Backmann, K. Conrath, S. Muyldermans, C. M Cardoso, and H. Leonhardt
JournalNat Methods
Volume3
Issue11
Pagination887-9
Date Published2006 Nov
ISSN1548-7091
KeywordsAnimals, Antibodies, Antigen-Antibody Reactions, Antigens, Camels, Epitopes, Green Fluorescent Proteins, Immunoglobulin Heavy Chains, Mitosis, S Phase
Abstract

We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

DOI10.1038/nmeth953
Alternate JournalNat. Methods
PubMed ID17060912