|Title||Targeting and tracing antigens in live cells with fluorescent nanobodies.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Rothbauer, U., K. Zolghadr, S. Tillib, D. Nowak, L. Schermelleh, A. Gahl, N. Backmann, K. Conrath, S. Muyldermans, C. M Cardoso, and H. Leonhardt|
|Date Published||2006 Nov|
|Keywords||Animals, Antibodies, Antigen-Antibody Reactions, Antigens, Camels, Epitopes, Green Fluorescent Proteins, Immunoglobulin Heavy Chains, Mitosis, S Phase|
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
|Alternate Journal||Nat. Methods|