Cloning and characterization of the dromedary (Camelus dromedarius) neonatal Fc receptor (drFcRn).

TitleCloning and characterization of the dromedary (Camelus dromedarius) neonatal Fc receptor (drFcRn).
Publication TypeJournal Article
Year of Publication2006
AuthorsKacskovics, I., B. Mayer, Z. Kis, L. V. Frenyó, Y. Zhao, S. Muyldermans, and L. Hammarström
JournalDev Comp Immunol
Volume30
Issue12
Pagination1203-15
Date Published2006
ISSN0145-305X
KeywordsAmino Acid Sequence, Animals, Animals, Newborn, Base Sequence, Camels, Cloning, Molecular, DNA, Complementary, DNA-Directed DNA Polymerase, Female, Histocompatibility Antigens Class I, Immunoglobulin alpha-Chains, Immunohistochemistry, Liver, Mammary Glands, Animal, Molecular Sequence Data, Phylogeny, Receptors, Fc, Reverse Transcriptase Polymerase Chain Reaction, RNA, Sequence Alignment
Abstract

The full length cDNA of the dromedary neonatal Fc receptor (drFcRn) alpha chain was isolated and found that it is similar to the neonatal Fc receptor (FcRn) of other species with a high overall similarity to ruminant FcRn alpha chains. The drFcRn/Fc contact residues are highly conserved and predicted to bind both conventional (IgG1) and heavy chain (IgG2a, IgG3) antibodies. Using immunohistochemistry, we detected its expression in the hepatocytes and in epithelial cells of portal bile ductuli and also in the mammary gland acini and ducti. Remarkably, Ser313, that was identified to be crucial for apical to basolateral transcytosis, is substituted in the drFcRn alpha chain. The full length of the dog and orangutan FcRn alpha chains was also identified from databases. Analyzing the phylogenetic relatedness of this gene we found that dromedary clustered together with artiodactyls, dog is located between artiodactyls and primates, where the orangutan was branched, reflecting the accepted evolutionary relationships.

DOI10.1016/j.dci.2006.02.006
Alternate JournalDev. Comp. Immunol.
PubMed ID16690125