Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells.

TitleHeavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells.
Publication TypeJournal Article
Year of Publication2003
AuthorsNguyen, V. Khong, X. Zou, M. Lauwereys, L. Brys, M. Br├╝ggemann, and S. Muyldermans
JournalImmunology
Volume109
Issue1
Pagination93-101
Date Published2003 May
ISSN0019-2805
KeywordsAnimals, Antibody Specificity, B-Lymphocytes, Camels, Feasibility Studies, Gene Rearrangement, Immunoglobulin G, Immunoglobulin Heavy Chains, Mice, Multiple Myeloma, Muramidase, Peptides, Species Specificity, Transfection
Abstract

Whereas functional heavy (H)-chain antibodies devoid of light (L)- chains account for about half of the circulating immunoglobulins in Camelidae, H-chain only antibodies (HCAbs) are not produced in other healthy mammals including rodents and humans. To test the feasibility of expressing single chain antibodies in the mouse, which on account of their small size and antigen-recognition properties would have a major impact on antibody engineering strategies, we constructed a rearranged dromedary H-chain gene encoding the immunoglobulin G2a (IgG2a) isotype with specificity for hen-egg lysozyme (HEL). This IgG2a H-chain gene was introduced into mouse myeloma cells not expressing endogenous immunoglobulin H- or L-chains. Unexpectedly the mouse cells processed and expressed the introduced H-chain as naturally occurring dromedary antibody. For this the first constant (C) region exon was proficiently removed from the recombinant H-chain transcript. This resulted in specific H-chain antibodies of the correct molecular weight (2 x 50 000 MW) secreted as disulfide-linked homodimers and displayed on the mouse cell surface as glycosyl-phosphatidyl-inositol-linked B-cell receptor. The results indicate that antibody expression and maturation without immunoglobulin L-chain is feasible and paves the way for the generation of transgenic single chain antibody repertoires.

Alternate JournalImmunology
PubMed ID12709022
PubMed Central IDPMC1782939