Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.

TitleCamel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.
Publication TypeJournal Article
Year of Publication2001
AuthorsK Conrath, E., M. Lauwereys, L. Wyns, and S. Muyldermans
JournalJ Biol Chem
Volume276
Issue10
Pagination7346-50
Date Published2001 Mar 9
ISSN0021-9258
KeywordsAmino Acid Sequence, Amylases, Animals, Antibodies, Antibodies, Bispecific, Biotinylation, Blotting, Western, Camelids, New World, Camels, Chromatography, Gel, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Escherichia coli, Kinetics, Molecular Sequence Data, Protein Structure, Tertiary, Recombinant Proteins, Sequence Homology, Amino Acid, Time Factors
Abstract

Single-domain antibodies against various antigens are isolated from the unique heavy-chain antibodies of immunized camels and llamas. These minimal sized binders are very robust and bind the antigen with high affinity in a monomeric state. We evaluated the feasibility to produce soluble, functional bispecific and bivalent antibodies in Escherichia coli with camel single-domain antibody fragments as building blocks. Two single-domain antibody fragments were tethered by the structural upper hinge of a natural antibody to generate bispecific molecules. This linker was chosen for its protease resistance in serum and its natural flexibility to reorient the upstream and downstream located domains. The expression levels, ease of purification, and the solubility of the recombinant proteins were comparable with those of the constituent monomers. The individual moieties fully retain the binding capacity and the binding characteristics within the recombinant bispecific constructs. The easy generation steps and the biophysical properties of these bispecific and bivalent constructs based on camel single-domain antibody fragments makes them particularly attractive for use in therapeutic or diagnostic programs.

DOI10.1074/jbc.M007734200
Alternate JournalJ. Biol. Chem.
PubMed ID11053416