The regulated expression of an intrabody produces a mutant phenotype in Drosophila.

TitleThe regulated expression of an intrabody produces a mutant phenotype in Drosophila.
Publication TypeJournal Article
Year of Publication1998
AuthorsG Gh, H., N. Devoogdt, A. Ghysen, P. De Baetselier, S. Muyldermans, and C. Dambly-Chaudière
JournalFEBS Lett
Volume437
Issue1-2
Pagination81-6
Date Published1998 Oct 16
ISSN0014-5793
KeywordsAnimals, Animals, Genetically Modified, DNA, Antisense, Drosophila, Drosophila Proteins, Gene Expression Regulation, Hot Temperature, Immunoglobulin Fragments, Mutation, Nerve Tissue Proteins, Paired Box Transcription Factors, Phenotype, Transcription Factors, Transfection
Abstract

Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in Drosophila, the gene poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic poxn-phenotype was produced in embryos and larvae following induction of expression of alpha-Poxn2 intrabody. The antisense approach was used as control. Parameters that can affect the success of intrabody technology are described.

Alternate JournalFEBS Lett.
PubMed ID9804176