The specific variable domain of camel heavy-chain antibodies is encoded in the germline.

TitleThe specific variable domain of camel heavy-chain antibodies is encoded in the germline.
Publication TypeJournal Article
Year of Publication1998
AuthorsNguyen, V. K., S. Muyldermans, and R. Hamers
JournalJ Mol Biol
Volume275
Issue3
Pagination413-8
Date Published1998 Jan 23
ISSN0022-2836
KeywordsAmino Acid Sequence, Animals, Base Sequence, Camels, Cloning, Molecular, Gene Rearrangement, Genes, Immunoglobulin, Immunoglobulin Heavy Chains, Immunoglobulin Variable Region, Molecular Sequence Data, Promoter Regions, Genetic, Protein Sorting Signals, Restriction Mapping
Abstract

The variable domains of the functional heavy-chain antibodies (VHHs) discovered in camels are related to the human VH subgroup III. They are nevertheless clearly distinguishable from the VHs of conventional four-chain immunoglobulins by the presence of important amino acid substitutions, located in the solvent-exposed surface normally covered by the variable domain of the light chain. The analysis of an unrearranged dromedary DNA library revealed that the specific VHH gene with its characteristic amino acid substitutions is encoded in the germline. Therefore, it is concluded that the VHHs do not arise through an ontogenic process of somatic hypermutation. The presence of putative DNA recombination signals that are more prevalent in the camel VHH, compared to the VH germline gene, might play a role in the formation and efficient expansion of the VHH repertoire.

DOI10.1006/jmbi.1997.1477
Alternate JournalJ. Mol. Biol.
PubMed ID9466919