A DNA sequence for positioning chromatosomes.

TitleA DNA sequence for positioning chromatosomes.
Publication TypeJournal Article
Year of Publication1996
AuthorsTravers, A. A., and S. Muyldermans
JournalJ Mol Biol
Volume257
Issue3
Pagination486-91
Date Published1996 Apr 5
ISSN0022-2836
KeywordsChromatin, Cloning, Molecular, DNA, Histones, Nucleosomes, Protein Conformation, Sequence Analysis
Abstract

We have analysed the sequences of 280 chromatosomal DNA molecules. In approximately half the clones, a short DNA sequence of the preferred form NGGR is located at one, but not both, of the termini of the cloned DNA. We show that the clones lacking this signal possess a substantially stronger rotational positioning signal than those that contain it. These results indicate that the sequence organisation of chromatosomal DNA is asymmetric with respect to the midpoint and imply that the sequence NGGR may play a direct role in positioning chromatosomes in condensed chromatin.

DOI10.1006/jmbi.1996.0178
Alternate JournalJ. Mol. Biol.
PubMed ID8648617