Differential association of linker histones H1 and H5 with telomeric nucleosomes in chicken erythrocytes.

TitleDifferential association of linker histones H1 and H5 with telomeric nucleosomes in chicken erythrocytes.
Publication TypeJournal Article
Year of Publication1994
AuthorsMuyldermans, S., J. De Jonge, L. Wyns, and A. A. Travers
JournalNucleic Acids Res
Volume22
Issue25
Pagination5635-9
Date Published1994 Dec 25
ISSN0305-1048
KeywordsAnimals, Base Sequence, Chickens, Erythrocytes, Histones, Liver, Molecular Sequence Data, Nucleosomes, Rats, Repetitive Sequences, Nucleic Acid, Telomere
Abstract

Rat liver telomeric DNA is organised into nucleosomes characterised by a shorter and more homogeneous average nucleosomal repeat than bulk chromatin as shown by Makarov et al. (1). The latter authors were unable to detect the association of any linker histone with the telomeric DNA. We have confirmed these observations but show that in sharp contrast chicken erythrocyte telomeric DNA is organised into nucleosomes whose spacing length and heterogeneity are indistinguishable from those of bulk chromatin. We further show that chicken erythrocyte telomeric chromatin contains chromatosomes which are preferentially associated with histone H1 relative to histone H5. This contrasts with bulk chromatin where histone H5 is the more abundant species. This observation strongly suggests that telomeric DNA condensed into nucleosome core particles has a higher affinity for H1 than H5. We discuss the origin of the discrimination of the lysine rich histones in terms of DNA sequence preferences, telomere nucleosome preferences and particular constraints of the higher order chromatin structure of telomeres.

Alternate JournalNucleic Acids Res.
PubMed ID7838716
PubMed Central IDPMC310127