Comparative filter binding study of H5 to nucleosome core particles, H1, H5 depleted chromatosomes and DNA fragments.

TitleComparative filter binding study of H5 to nucleosome core particles, H1, H5 depleted chromatosomes and DNA fragments.
Publication TypeJournal Article
Year of Publication1988
AuthorsPennings, S., S. Muyldermans, and L. Wyns
JournalMol Biol Rep
Volume13
Issue4
Pagination191-6
Date Published1988-1989
ISSN0301-4851
KeywordsAnimals, Cell Nucleus, Chickens, DNA, Erythrocytes, Histones, Micropore Filters, Nucleosomes, Polyethyleneimine
Abstract

The filter-binding technique with PEI treated glass fiber is used to study the interaction of histone H5 to core particles, chromatosomes and DNA derived from it. By working at very low concentrations of interacting particles we are able to study the effective binding process independent of interfering insoluble complexes. The interactions are characterized by a very high affinity. An intrinsically higher affinity of H5 for cores and chromatosomes versus chromatosome derived DNA is demonstrated. Both chromatosomes and DNA derived from these bind about twice the amount as compared to core particles, which saturate at about one H5 per core particle.

Alternate JournalMol. Biol. Rep.
PubMed ID3272346