|Title||Putative regulatory sequences for the transcription of mini-exons in Trypanosoma brucei as revealed by S1 sensitivity.|
|Publication Type||Journal Article|
|Year of Publication||1985|
|Authors||Michiels, F., S. Muyldermans, R. Hamers, and G. Matthyssens|
|Keywords||Animals, Base Sequence, Cloning, Molecular, Endonucleases, Genes, Regulator, Plasmids, Repetitive Sequences, Nucleic Acid, RNA, Messenger, Single-Strand Specific DNA and RNA Endonucleases, Transcription, Genetic, Trypanosoma brucei brucei|
The 35-nucleotide (nt) mini-exon found at the 5' end of most Trypanosoma brucei mRNAs is encoded as part of a tandem 1.35-kb repeat in genomic DNA. We cloned this DNA and identified an S1-sensitive site in supercoiled plasmids containing mini-exon repeats. This site is situated on a poly(dA-dT) stretch that is variable in length in different copies of repeat. Poly(dA-dT) is capable of forming abnormal DNA helix configurations, some of which are induced by supercoiling. The S1 site may have a role in regulation of mini-exon transcription.