The structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.

TitleThe structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.
Publication TypeJournal Article
Year of Publication1981
AuthorsHoussier, C., I. Lasters, S. Muyldermans, and L. Wyns
JournalNucleic Acids Res
Volume9
Issue21
Pagination5763-84
Date Published1981 Nov 11
ISSN0305-1048
KeywordsAnimals, Birefringence, Chickens, DNA, Electrochemistry, Erythrocytes, Liver, Macromolecular Substances, Nucleic Acid Conformation, Nucleosomes, Rats
Abstract

The spatial organization of nucleosomes and linker DNA in dinucleosomes and oligonucleosomes of various chain lengths has been investigated through electric dichroism, birefringence and relaxation times measurements at low ionic strengths (0.5 to 2.2 mM). From the negative dichroism observed for all the samples, it is concluded that the nucleosome subunits in the oligonucleosome chain must lie with their disc planes closely parallel to the fibre axis. The large increase of the negative dichroism of dinucleosomes upon Hl removal is interpreted by the unwinding of the DNA tails and the internucleosomal segment. All the samples displayed, under bipolar pulses, a predominantly induced orientation mechanism.

Alternate JournalNucleic Acids Res.
PubMed ID7312629
PubMed Central IDPMC327559