Differences in rearrangements of H1 and H5 in chicken erythrocyte chromatin.

TitleDifferences in rearrangements of H1 and H5 in chicken erythrocyte chromatin.
Publication TypeJournal Article
Year of Publication1981
AuthorsLasters, I., S. Muyldermans, L. Wyns, and R. Hamers
JournalBiochemistry
Volume20
Issue5
Pagination1104-10
Date Published1981 Mar 3
ISSN0006-2960
KeywordsAnimals, Centrifugation, Density Gradient, Chemical Precipitation, Chickens, Chromatin, Erythrocytes, Histones, Molecular Weight
Abstract

H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the chicken erythrocyte specific lysine-rich histone H5 does not show any of these dynamic properties. This difference in mobility of H1 and H5 also manifests itself in the selective extraction of H1 from H1, H5 containing polynucleosomes by the cation-exchange resin AG 50W-X2 at 80 mM NaCl.

Alternate JournalBiochemistry
PubMed ID7225320