Title | Upon the observation of superbeads in chromatin. |
Publication Type | Journal Article |
Year of Publication | 1980 |
Authors | Muyldermans, S., I. Lasters, L. Wyns, and R. Hamers |
Journal | Nucleic Acids Res |
Volume | 8 |
Issue | 10 |
Pagination | 2165-72 |
Date Published | 1980 May 24 |
ISSN | 0305-1048 |
Keywords | Animals, Centrifugation, Density Gradient, Chromatin, Magnesium, Nucleosomes, Rats, Ribonucleoproteins, Solubility |
Abstract | There exist some indications that nucleases recognize "superbeads" in chromatin. We show that a chromatin extract of rat liver which contains so-called "superbead"-peaks can be separated in a Mg++ soluble and a Mg++ insoluble fraction. The Mg++ insoluble fraction contains the full complement of histones and the expected DNA fragments, but has lost the characteristic peaks in sucrosegradient profiles. These discrete peaks are found in the Mg2+ soluble fraction of the chromatin extract. We give evidence that these peaks are RNP particles on the basis of their protein- and nucleic acid contents. |
Alternate Journal | Nucleic Acids Res. |
PubMed ID | 7433089 |
PubMed Central ID | PMC324070 |
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