Upon the observation of superbeads in chromatin.

TitleUpon the observation of superbeads in chromatin.
Publication TypeJournal Article
Year of Publication1980
AuthorsMuyldermans, S., I. Lasters, L. Wyns, and R. Hamers
JournalNucleic Acids Res
Volume8
Issue10
Pagination2165-72
Date Published1980 May 24
ISSN0305-1048
KeywordsAnimals, Centrifugation, Density Gradient, Chromatin, Magnesium, Nucleosomes, Rats, Ribonucleoproteins, Solubility
Abstract

There exist some indications that nucleases recognize "superbeads" in chromatin. We show that a chromatin extract of rat liver which contains so-called "superbead"-peaks can be separated in a Mg++ soluble and a Mg++ insoluble fraction. The Mg++ insoluble fraction contains the full complement of histones and the expected DNA fragments, but has lost the characteristic peaks in sucrosegradient profiles. These discrete peaks are found in the Mg2+ soluble fraction of the chromatin extract. We give evidence that these peaks are RNP particles on the basis of their protein- and nucleic acid contents.

Alternate JournalNucleic Acids Res.
PubMed ID7433089
PubMed Central IDPMC324070