Histone H1 can be removed selectively from chicken erythrocyte chromatin at near physiological conditions.

TitleHistone H1 can be removed selectively from chicken erythrocyte chromatin at near physiological conditions.
Publication TypeJournal Article
Year of Publication1980
AuthorsMuyldermans, S., I. Lasters, and L. Wyns
JournalNucleic Acids Res
Volume8
Issue4
Pagination731-9
Date Published1980 Feb 25
ISSN0305-1048
KeywordsAnimals, Chickens, Chromatin, Erythrocytes, Histones, Ion Exchange Resins, Nucleosomes, Solubility
Abstract

Histone H1 was depleted selectively from chicken erythrocyte polynucleosomes, without any detectable concomitant loss of H5 or core particle histones. The depletion is performed with ion exchange resin at low ionic strength (80 mM NaCl). The nucleosomes did not slide during the procedure. In contrast to the native chromatin, H1 depleted polynucleosomes are completely soluble in the 5--600 mM NaCl range.

Alternate JournalNucleic Acids Res.
PubMed ID7433115
PubMed Central IDPMC327306