Title | Purification and crystallization of Phd, the antitoxin of the phd/doc operon. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Garcia-Pino, A., Y. Sterckx, G. Vandenbussche, and R. Loris |
Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
Volume | 66 |
Issue | Pt 2 |
Pagination | 167-71 |
Date Published | 2010 Feb 1 |
Type of Article | ta |
ISSN | 1744-3091 |
Keywords | Antitoxins, Bacteriophage P1, Mass Spectrometry, Operon, Viral Proteins, X-Ray Diffraction |
Abstract | The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein. |
DOI | 10.1107/S1744309109051550 |
Alternate Journal | Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. |
PubMed ID | 20124714 |
PubMed Central ID | PMC2815684 |
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