|Title||Combining pattern discovery and discriminant analysis to predict gene co-regulation.|
|Publication Type||Journal Article|
|Year of Publication||2004|
|Authors||Simonis, N., Wodak S. J., Cohen G. N., and van Helden J.|
|Date Published||2004 Oct 12|
|Keywords||Algorithms, Computer Simulation, Discriminant Analysis, Gene Expression Regulation, Genes, Regulator, Models, Genetic, Models, Statistical, Pattern Recognition, Automated, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Analysis, DNA, Transcription Factors|
MOTIVATION: Several pattern discovery methods have been proposed to detect over-represented motifs in upstream sequences of co-regulated genes, and are for example used to predict cis-acting elements from clusters of co-expressed genes. The clusters to be analyzed are often noisy, containing a mixture of co-regulated and non-co-regulated genes. We propose a method to discriminate co-regulated from non-co-regulated genes on the basis of counts of pattern occurrences in their non-coding sequences.METHODS: String-based pattern discovery is combined with discriminant analysis to classify genes on the basis of putative regulatory motifs.RESULTS: The approach is evaluated by comparing the significance of patterns detected in annotated regulons (positive control), random gene selections (negative control) and high-throughput regulons (noisy data) from the yeast Saccharomyces cerevisiae. The classification is evaluated on the annotated regulons, and the robustness and rejection power is assessed with mixtures of co-regulated and random genes.