Title | Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of Staphylococcus aureus thioredoxin. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Roos, G., E. Brosens, K. Wahni, A. Desmyter, S. Spinelli, L. Wyns, J. Messens, and R. Loris |
Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
Volume | 62 |
Issue | Pt 12 |
Pagination | 1255-8 |
Date Published | 2006 Dec 1 |
ISSN | 1744-3091 |
Keywords | Crystallization, Crystallography, X-Ray, Mutagenesis, Site-Directed, Staphylococcus aureus, Thioredoxins |
Abstract | The P31T mutant of Staphylococcus aureus thioredoxin crystallizes spontaneously in space group P2(1)2(1)2(1), with unit-cell parameters a = 41.7, b = 49.5, c = 55.6 A. The crystals diffract to 2.2 A resolution. Isomorphous crystals of wild-type thioredoxin as well as of other point mutants only grow when seeded with the P31T mutant. These results suggest seeding as a valuable tool complementing surface engineering for proteins that are hard to crystallize. |
DOI | 10.1107/S1744309106047075 |
Alternate Journal | Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. |
PubMed ID | 17142910 |
PubMed Central ID | PMC2225371 |
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