Title | Inactivated Salmonella expressing the receptor-binding domain of bacterial adhesins elicit antibodies inhibiting hemagglutination. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Van Gerven, N., H. De Greve, and J-P. Hernalsteens |
Journal | Vet Microbiol |
Volume | 131 |
Issue | 3-4 |
Pagination | 369-75 |
Date Published | 2008 Oct 15 |
ISSN | 0378-1135 |
Keywords | Adhesins, Bacterial, Animals, Antibodies, Bacterial, Erythrocytes, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Hemagglutination, Mice, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, Salmonella |
Abstract | We examined the potential of inactivated Salmonella strains to induce protective antibodies against two adhesins of pathogenic Escherichia coli. The receptor-binding domains of the F17a-G adhesin of F17a fimbriae and of the FimH adhesin of type 1 fimbriae were fused to the translocator domain of the autotransporter AIDA-I. An IgG response against F17a-G or FimH was induced after immunization of mice with acetone-inactivated Salmonella displaying the corresponding fimbrial receptor-binding domain. These sera inhibit in vitro agglutination of erythrocytes by E. coli carrying these fimbriae. Our results demonstrate that induced and subsequently acetone-inactivated Salmonella are useful delivery vehicles for the stimulation of an IgG antibody response against heterologous antigens. |
DOI | 10.1016/j.vetmic.2008.04.001 |
Alternate Journal | Vet. Microbiol. |
PubMed ID | 18502056 |
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