Title | Identification of an ATP-binding cassette transporter for export of the O-antigen across the inner membrane in Rhizobium etli based on the genetic, functional, and structural analysis of an lps mutant deficient in O-antigen. |
Publication Type | Journal Article |
Year of Publication | 2001 |
Authors | Lerouge, I., T. Laeremans, C. Verreth, J. Vanderleyden, C. Van Soom, A. Tobin, and R. W. Carlson |
Journal | J Biol Chem |
Volume | 276 |
Issue | 20 |
Pagination | 17190-8 |
Date Published | 2001 May 18 |
ISSN | 0021-9258 |
Keywords | Amino Acid Sequence, ATP-Binding Cassette Transporters, Bacteria, Biological Transport, Cell Membrane, Cytoplasm, Lipopolysaccharides, Molecular Sequence Data, O Antigens, Open Reading Frames, Phylogeny, Protein Transport, Rhizobium, Sequence Alignment, Sequence Homology, Amino Acid |
Abstract | For O-antigen lipopolysaccharide (LPS) synthesis in bacteria, transmembrane migration of undecaprenyl pyrophosphate-bound O-antigen oligosaccharide subunits or polysaccharide occurs before ligation to the core region of the LPS molecule. In this study, we identified by mutagenesis an ATP-binding cassette transporter in Rhizobium etli CE3 that is likely responsible for the translocation of the O-antigen across the inner plasma membrane. Mutant FAJ1200 LPS lacks largely the O-antigen, as shown by SDS-polyacrylamide gel electrophoresis and confirmed by immunoblot analysis. Furthermore, LPS isolated from FAJ1200 is totally devoid of any O-chain glycosyl residues and contains only those glycosyl residues that can be expected for the inner core region. The membrane component and the cytoplasmic ATP-binding component of the ATP-binding cassette transporter are encoded by wzm and wzt, respectively. The Tn5 transposon in mutant FAJ1200 is inserted in the wzm gene. This mutation resulted in an Inf- phenotype in bean plants. |
DOI | 10.1074/jbc.M101129200 |
Alternate Journal | J. Biol. Chem. |
PubMed ID | 11279176 |
Grant List | GM39583 / GM / NIGMS NIH HHS / United States |
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