Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya.

TitleCrystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya.
Publication TypeJournal Article
Year of Publication2006
AuthorsAzarkan, M., A. Garcia-Pino, R. Dibiani, L. Wyns, R. Loris, and D. Baeyens-Volant
JournalActa Crystallogr Sect F Struct Biol Cryst Commun
Volume62
IssuePt 12
Pagination1239-42
Date Published2006 Dec 1
ISSN1744-3091
KeywordsCarica, Crystallization, Crystallography, X-Ray, Peptides, Plant Proteins, Trypsin Inhibitors
Abstract

A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 A and belongs to space group P3(1) or P3(2). This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 A. Crystal form II, which was grown in the presence of Co2+, diffracts to a resolution of 1.7 A and belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 A.

DOI10.1107/S1744309106046367
Alternate JournalActa Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
PubMed ID17142906
PubMed Central IDPMC2225369