Phaseolus vulgaris recognizes Azorhizobium caulinodans Nod factors with a variety of chemical substituents.

TitlePhaseolus vulgaris recognizes Azorhizobium caulinodans Nod factors with a variety of chemical substituents.
Publication TypeJournal Article
Year of Publication1999
AuthorsLaeremans, T., C. Snoeck, J. Mariën, C. Verreth, E. Martínez-Romero, J. C. Promé, and J. Vanderleyden
JournalMol Plant Microbe Interact
Volume12
Issue9
Pagination820-4
Date Published1999 Sep
ISSN0894-0282
KeywordsBase Sequence, Conjugation, Genetic, DNA Primers, Fabaceae, Genes, Bacterial, Lipopolysaccharides, Mutation, Plants, Medicinal, Rhizobium
Abstract

Phaseolus vulgaris is a promiscuous host plant that can be nodulated by many different rhizobia representing a wide spectrum of Nod factors. In this study, we introduced the Rhizobium tropici CFN299 Nod factor sulfation genes nodHPQ into Azorhizobium caulinodans. The A. caulinodans transconjugants produce Nod factors that are mostly if not all sulfated and often with an arabinosyl residue as the reducing end glycosylation. Using A. caulinodans mutant strains, affected in reducing end decorations, and their respective transconjugants in a bean nodulation assay, we demonstrated that bean nodule induction efficiency, in decreasing order, is modulated by the Nod factor reducing end decorations fucose, arabinose or sulfate, and hydrogen.

DOI10.1094/MPMI.1999.12.9.820
Alternate JournalMol. Plant Microbe Interact.
PubMed ID10494633