Nanobodies

Nanobody production and applications

Interactions between Metal-binding Domains Modulate Intracellular Targeting of Cu(I)-ATPase ATP7B, as Revealed by Nanobody Binding.

Huang, Y., S. Nokhrin, G. Hassanzadeh-Ghassabeh, C. H. Yu, H. Yang, A. N. Barry, M. Tonelli, J. L. Markley, S. Muyldermans, O. Y. Dmitriev, et al., "Interactions between Metal-binding Domains Modulate Intracellular Targeting of Cu(I)-ATPase ATP7B, as Revealed by Nanobody Binding.", J Biol Chem, vol. 289, issue 47, pp. 32682-93, 2014 Nov 21.

TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers.

Jovčevska, I., N. Zupanec, N. Kočevar, D. Cesselli, N. Podergajs, C. Limbaeck Stokin, M. P. Myers, S. Muyldermans, G. Hassanzade Ghassabeh, H. Motaln, et al., "TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers.", PLoS One, vol. 9, issue 11, pp. e113688, 2014.

Monitoring liver macrophages using nanobodies targeting Vsig4: Concanavalin A induced acute hepatitis as paradigm.

Zheng, F., N. Devoogdt, A. Sparkes, Y. Morias, C. Abels, B. Stijlemans, T. Lahoutte, S. Muyldermans, P. De Baetselier, S. Schoonooghe, et al., "Monitoring liver macrophages using nanobodies targeting Vsig4: Concanavalin A induced acute hepatitis as paradigm.", Immunobiology, vol. 220, issue 2, pp. 200-9, 2015 Feb.

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.

Ehrnstorfer, I. A., E. R. Geertsma, E. Pardon, J. Steyaert, and R. Dutzler, "Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.", Nat Struct Mol Biol, vol. 21, issue 11, pp. 990-6, 2014 Nov.

Production, crystallization and preliminary X-ray diffraction of the Gαs α-helical domain in complex with a nanobody.

Triest, S., A. Wohlkonig, E. Pardon, and J. Steyaert, "Production, crystallization and preliminary X-ray diffraction of the Gαs α-helical domain in complex with a nanobody.", Acta Crystallogr F Struct Biol Commun, vol. 70, issue Pt 11, pp. 1504-7, 2014 Nov.

Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain.

Nunes-Silva, S., S. Gangnard, M. Vidal, A. Vuchelen, S. Dechavanne, S. Chan, E. Pardon, J. Steyaert, S. Ramboarina, A. Chêne, et al., "Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain.", Sci Rep, vol. 4, pp. 7373, 2014 Dec 09.

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